Galactose metabolic genes in yeast respond to a ratio of galactose and glucose Supporting Information I. Strain construction
ثبت نشده
چکیده
I. Strain construction The strains used in this study are listed in Table S1. The wild type strain background used for this paper was S288C, specifically FY4 (MAT a), FY5 (MAT α), and BY4741 (1). Multiple derivatives of this strain were created by standard transformation protocols. For example, SLYH14 was created by transforming a NotI digest of plasmid SLVD02, a HO-GAL1pr-YFP-hphNT1-HO integration cassette into FY4 and selecting for hygromycin resistance. FY5 was transformed with a NotI digest of SLVA06, a HO-TDH3pr-mCherry-natMX4-HO integration cassette, and selected for clonNAT to create SLYH15. SLYH9 was then generated by mating SLYH14 and SLYH15, and selecting for hygromycin and clonNAT resistant colonies. The strains BC1887 and YJM978 (2) were also used in this study. Strains were confirmed by colony PCR and a fluorescent readout.
منابع مشابه
Galactose metabolic genes in yeast respond to a ratio of galactose and glucose.
Natural environments are filled with multiple, often competing, signals. In contrast, biological systems are often studied in "well-controlled" environments where only a single input is varied, potentially missing important interactions between signals. Catabolite repression of galactose by glucose is one of the best-studied eukaryotic signal integration systems. In this system, it is believed ...
متن کاملTiming and Variability of Galactose Metabolic Gene Activation Depend on the Rate of Environmental Change
Modulation of gene network activity allows cells to respond to changes in environmental conditions. For example, the galactose utilization network in Saccharomyces cerevisiae is activated by the presence of galactose but repressed by glucose. If both sugars are present, the yeast will first metabolize glucose, depleting it from the extracellular environment. Upon depletion of glucose, the genes...
متن کاملTransformation and expression of Penicillium funicolusum glucose oxidase gene in yeast
Glucose oxidase is an important enzyme hydrolyzing for its hydrolyzing activity on glucos. It possesses and has a wide board of applications in different industries such as bakery, pharmaceutical, plant pathology and biosensors. In this study, yeast (Saccharomyces cerevisiae) was transformed successfully by the glucose oxidase gene (gox) obtained from Penicillium funicolusum. The secreted gluco...
متن کاملImprovement of galactose uptake in Saccharomyces cerevisiae through overexpression of phosphoglucomutase: example of transcript analysis as a tool in inverse metabolic engineering.
Through genome-wide transcript analysis of a reference strain and two recombinant Saccharomyces cerevisiae strains with different rates of galactose uptake, we obtained information about the global transcriptional response to metabolic engineering of the GAL gene regulatory network. One of the recombinant strains overexpressed the gene encoding the transcriptional activator Gal4, and in the oth...
متن کاملMetabolic engineering of a Saccharomyces cerevisiae strain capable of simultaneously utilizing glucose and galactose to produce enantiopure (2R,3R)-butanediol.
2,3-Butanediol (BDO) is an important chemical with broad industrial applications and can be naturally produced by many bacteria at high levels. However, the pathogenicity of these native producers is a major obstacle for large scale production. Here we report the engineering of an industrially friendly host, Saccharomyces cerevisiae, to produce BDO at high titer and yield. By inactivation of py...
متن کامل